Posted in Business & Finance, Government & Military, Information Technology, Organizations, Pop Culture DOTA stands for Defense of the Ancients (gaming) DOTA stands for Depending on the Artist (trope) DOTA stands for Doscientos Ocho Transporte Automotor (Buenos Aires bus line; Buenos Aires, Argentina) DOTA stands for Download over the Air (Wavecom Wireless) DOTA stands for Declaration of the Americas on Diabetes DOTA stands for Département d’Optique Théorique et Appliquée (French: Theoretical and Applied Optics Department)